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http://hdl.handle.net/123456789/2592
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Название: | Interaction of ROS and Nitric Oxide during Induction of Heat Resistance of Wheat Seedlings by Hydrogen Sulfide Donor |
Авторы: | Karpets Y.V. Kolupaev Y.E. Lugovaya A.A. Shvydenko M.V. Shkliarevskyi M.A. Yastreb T.O. |
Ключевые слова: | Triticum aestivum hydrogen sulfide reactive oxygen species nitric oxide nitrate reductase heat resistance |
Дата публикации: | 2020 |
Библиографическое описание: | Karpets, Y.V., Kolupaev, Y.E., Lugovaya, A.A., Shvydenko, M.V., Shkliarevskyi, M.A., Yastreb, T.O. Functional Interaction of ROS and Nitric Oxide during Induction of Heat Resistance of Wheat Seedlings by Hydrogen Sulfide Donor. Russian Journal of Plant Physiology, 2020, 67(4) : 653-660 |
Серия/номер: | лісівництво; |
Аннотация: | The participation of reactive oxygen species (ROS) and nitric oxide (NO), and also enzymatic systems
generating them, in the development of heat resistance of wheat (Triticum aestivum L.) seedlings,
induced by the hydrogen sulfide (H2S) donor sodium hydrosulfide (NaHS), has been studied. It was found that
24-h pretreatment of seedlings with 0.1–1 mM NaHS increased their survival after the subsequent 10-min
damaging heating at 45°C. The content of hydrogen peroxide and nitric oxide increased together with the
nitrate reductase (NR) activity in the seedling roots within the first 4 h of their treatment with the H2S donor.
The rise in the NO level significantly suppressed by the inhibitor of NR sodium tungstate but not the inhibitor
of NO synthase (NG-nitro-L-arginine methyl ester, L-NAME). The hydrogen peroxide scavenger dimethylthiourea
(DMTU) and the NADPH oxidase inhibitor imidazole abolished the increase in NR activity and
NO content in the roots. However, the nitric oxide scavenger (2-phenyl-4,4,5,5-tetramethylimidazoline-1-
oxyl-3-oxide, PTIO) and the inhibitors of the NO-producing enzymes only weakly influenced the increase
in H2O2 content caused by the root treatment with sodium hydrosulfide. The NaHS-induced rise in the seedling
heat resistance was eliminated by both ROS antagonists (DMTU and imidazole) and NO antagonists
(PTIO and tungstate). It is concluded that the boost in the wheat seedling heat resistance, which is caused by
exogenous hydrogen sulfide, is mediated by the increased ROS generation, followed by NR activation, and
resultant rise in the level of nitric oxide produced by this enzyme. |
URI: | http://hdl.handle.net/123456789/2592 |
Располагается в коллекциях: | Кафедра лісівництва
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